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M9460569.TXT
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1994-06-25
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Document 0569
DOCN M9460569
TI Multiple effects of CD4 CDR3-related peptide derivatives showing
anti-HIV-1 activity on HIV-1 gp120 functions.
DT 9408
AU Ohki K; Kimura T; Jones IM; Morita F; Ikuta K; Section of Serology,
Hokkaido University, Sapporo, Japan.
SO Vaccine. 1994 Mar;12(4):343-50. Unique Identifier : AIDSLINE
MED/94233850
AB The interaction of the human immunodeficiency virus type 1 (HIV-1)
envelope glycoprotein gp120 with CD4 CDR3-related peptide derivatives
showing anti-HIV-1 activity has been studied. Conformational changes in
gp120, which could affect its interaction with CD4 and its shedding from
virions, were detected by fluorescence spectrum analysis of tryptophan
residues after addition of peptide representative of the CD4
CDR3-related region, but not the CD4 CDR2-related region. Interestingly,
the addition of scrambled peptide, S1 (with altered amino acid sequence
compared with the native CDR3-related peptide but unaltered overall
composition), which we recently showed to have stronger anti-HIV-1
activity than the original CDR3-related peptide, had no effects on the
conformational change in gp120 or on its interaction with CD4 and its
shedding from HIV-1 virions. However, all of the CDR3-related peptides,
including S1, showed blocking effects on the binding of antibodies
against gp120 V3 loop and C-terminus regions. Thus, we concluded that
there were at least two separable activities of the CDR3-related
peptides in anti-HIV-1 activity, i.e. induction of conformational
changes in gp120, which could affect its binding to CD4 and to gp41 (as
observed in native CDR3-related peptides), and inactivation of V3 loop
and C-terminus regions in gp120 (as observed in all of the CDR3-related
peptides, including S1).
DE Amino Acid Sequence Antibodies, Monoclonal/IMMUNOLOGY Antigens,
CD4/*IMMUNOLOGY Cell Line Enzyme-Linked Immunosorbent Assay Flow
Cytometry Human HIV Antibodies/IMMUNOLOGY HIV Envelope Protein
gp120/*IMMUNOLOGY/METABOLISM HIV-1/*IMMUNOLOGY Immunoglobulin Variable
Region/IMMUNOLOGY Molecular Sequence Data Peptide
Fragments/*IMMUNOLOGY Protein Conformation Support, Non-U.S. Gov't
Tumor Cells, Cultured JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).